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Human Endostatin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Endostatin in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Collagen alpha-1(XVIII) chain, COL18A1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 10 pg/mL
13.7 - 10000 pg/mL
Microplate
Abcam's Human Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endostatin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Endostatin ELISA Kit (ab184864) from our SimpleStep ELISA® range.
Optimisation may be required with urine samples.
Representative Standard Curve using ab100508
Representative Standard Curve using ab100508
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