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Human G-CSF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human G-CSF in Human in Biofluids, Cell Culture Media samples.
隐藏别名
C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 1 pg/mL
0.69 - 500 pg/mL
Microplate
Abcam's G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
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-20°C
Representative standard curve using ab100524
Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).
Representative standard curve using ab100524
G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).
Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).
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