| 存储条件 |
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| Product Name | Cdc37 [JJ087-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC 37 antibody Cdc37 antibody CDC37 cell division cycle 37 homolog antibody CDC37 cell division cycle 37 S cerevisiae homolog antibody CDC37 cell division cycle 37, S cerevisiae, homolog of antibody Cdc37 homolog antibody CDC37 protein antibody CDC37_HUMAN antibody CDC37A antibody cell division cycle 37 antibody Cell division cycle 37 homolog antibody Hsp90 chaperone protein kinase targeting subunit antibody Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody Hsp90 chaperone protein kinase-targeting subunit antibody Hsp90 co chaperone Cdc37 antibody Hsp90 co-chaperone Cdc37 antibody p50 antibody p50Cdc37 antibody S cerevisiae hypothetical protein CDC37 antibody |
| Product description | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CDC 37 antibody Cdc37 antibody CDC37 cell division cycle 37 homolog antibody CDC37 cell division cycle 37 S cerevisiae homolog antibody CDC37 cell division cycle 37 S cerevisiae homolog of antibody Cdc37 homolog antibody CDC37 protein antibody CDC37_HUMAN antibody CDC37A antibody cell division cycle 37 antibody Cell division cycle 37 homolog antibody Hsp90 chaperone protein kinase targeting subunit antibody Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody Hsp90 chaperone protein kinase-targeting subunit antibody Hsp90 co chaperone Cdc37 antibody Hsp90 co-chaperone Cdc37 antibody p50 antibody p50Cdc37 antibody S cerevisiae hypothetical protein CDC37 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Cdc37 on different lysates using anti-Cdc37 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: A431 Lane 3: K562
Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cdc37 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Cdc37 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with Cdc37 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Jurkat, A431, K562, mouse placenta tissue, mouse liver tissue. |
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| Application Notes | WB:1:1,000-1:5,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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