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| Product Name | CD66A [7B9G3] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen CD66 antibody BGP 1 antibody BGP antibody BGP-1 antibody BGPI antibody Biliary glycoprotein 1 antibody Biliary glycoprotein adhesion molecule antibody Biliary glycoprotein antibody Carcinoembryonic antigen related cell adhesion molecule 1 antibody carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody Carcinoembryonic antigen-related cell adhesion molecule 1 antibody CD66a antibody CD66a antigen antibody CEACAM1 antibody CEAM1_HUMAN antibody meconium antigen 100 antibody |
| Product description | This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. |
| Immunogen | Purified recombinant fragment of human CD66A (AA: extra 65-201) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Antigen CD66 antibody BGP 1 antibody BGP antibody BGP-1 antibody BGPI antibody Biliary glycoprotein 1 antibody Biliary glycoprotein adhesion molecule antibody Biliary glycoprotein antibody Carcinoembryonic antigen related cell adhesion molecule 1 antibody carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody Carcinoembryonic antigen-related cell adhesion molecule 1 antibody CD66a antibody CD66a antigen antibody CEACAM1 antibody CEAM1_HUMAN antibody meconium antigen 100 antibody |
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| Molecular Weight(MW) | 57.6kDa |
| Cellular Localization | Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact. |
| SwissProt ID | P13688 |
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Application
Western blot analysis of CD66A against human CD66A (AA: extra 65-201) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-85 against HEK293 (1) and CD66A (AA: extra 65-201)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD66A against HepG2 (1), SK-MES-1 (2), Hela (3), and A549 (4) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-CD66A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-85, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-CD66A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-85, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD66A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-85, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HepG2, SK-MES-1, Hela, and A549 cell Hela cellslung cancercervical cancer |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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