14 3 3 gamma antibody 14 3 3 protein gamma antibody 14 3 3 protein gamma subtype antibody 14 3 3gamma antibody 14-3-3 protein gamma antibody 1433G_HUMAN antibody 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody KCIP 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein kinase C inhibitor protein 1 antibody Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody Ywhag antibody
Product description
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities.
Immunogen
recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Recombinant rabbit
Tested Applications
WBFC
WB:1:1,000-1:2,000 FC:1:10-1:50
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Target Information
Alternative Names
14 3 3 gamma antibody 14 3 3 protein gamma antibody 14 3 3 protein gamma subtype antibody 14 3 3gamma antibody 14-3-3 protein gamma antibody 1433G_HUMAN antibody 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody KCIP 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein kinase C inhibitor protein 1 antibody Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody Ywhag antibody
Fig1: Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: A431 Lane 3: Hela
Application
Fig2: Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the sec?皪?皫??皩?羉瘍
Positive Control
A431, Hela, 293T.
Application Notes
WB:1:1,000-1:2,000 FC:1:10-1:50
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
