| 存储条件 |
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| Product Name | FOLR1 [2G5C12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | adult antibody Adult folate binding protein antibody Adult folate-binding protein antibody FBP antibody Folate Binding Protein antibody Folate Receptor 1 Adult antibody Folate receptor 1 antibody Folate Receptor 1 Precursor antibody Folate receptor adult antibody Folate receptor alpha antibody Folate receptor antibody FOLR antibody FOLR1 antibody FOLR1_HUMAN antibody FR alpha antibody FR-alpha antibody FRalpha antibody KB cells FBP antibody MOV18 antibody Ovarian cancer associated antigen antibody Ovarian tumor associated antigen antibody Ovarian tumor associated antigen MOv18 antibody Ovarian tumor-associated antigen MOv18 antibody |
| Product description | The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. |
| Immunogen | Purified recombinant fragment of human FOLR1 (AA: 41-227) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | adult antibody Adult folate binding protein antibody Adult folate-binding protein antibody FBP antibody Folate Binding Protein antibody Folate Receptor 1 Adult antibody Folate receptor 1 antibody Folate Receptor 1 Precursor antibody Folate receptor adult antibody Folate receptor alpha antibody Folate receptor antibody FOLR antibody FOLR1 antibody FOLR1_HUMAN antibody FR alpha antibody FR-alpha antibody FRalpha antibody KB cells FBP antibody MOV18 antibody Ovarian cancer associated antigen antibody Ovarian tumor associated antigen antibody Ovarian tumor associated antigen MOv18 antibody Ovarian tumor-associated antigen MOv18 antibody |
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| Molecular Weight(MW) | 29.8kDa |
| Cellular Localization | Cell membrane. Secreted. |
| SwissProt ID | P15328 |
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Application
Western blot analysis of FOLR1 against human FOLR1 (AA: 41-227) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of FOLR1 against HEK293 (1) and FOLR1 (AA: 41-227)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of FOLR1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-FOLR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-72, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using anti-FOLR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-72, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of FOLR1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-72, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells, cervical cancer tissues, rectum cancer tissues |
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| Application Notes | WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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