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| Product Name | CD183 [5C10E6] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | C-X-C chemokine receptor type 3 antibody CD 183 antibody CD182 antibody CD183 antibody Chemokine (C X C motif) receptor 3 antibody Chemokine (C X C) receptor 3 antibody Chemokine CXC Motif Receptor 3 antibody CKR L2 antibody CKR-L2 antibody CKRL2 antibody CMKAR3 antibody CXC-R3 antibody CXCR-3 antibody CXCR3 antibody CXCR3_HUMAN antibody G Protein Coupled Receptor 9 antibody G protein-coupled receptor 9 antibody GPR9 antibody Interferon-inducible protein 10 receptor antibody IP-10 receptor antibody IP10 antibody IP10 R antibody IP10 receptor antibody IP10-R antibody IP10R antibody Mig R antibody Mig receptor antibody Mig-R antibody MIGR antibody |
| Product description | This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). |
| Immunogen | Purified recombinant fragment of human CD183 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C-X-C chemokine receptor type 3 antibody CD 183 antibody CD182 antibody CD183 antibody Chemokine (C X C motif) receptor 3 antibody Chemokine (C X C) receptor 3 antibody Chemokine CXC Motif Receptor 3 antibody CKR L2 antibody CKR-L2 antibody CKRL2 antibody CMKAR3 antibody CXC-R3 antibody CXCR-3 antibody CXCR3 antibody CXCR3_HUMAN antibody G Protein Coupled Receptor 9 antibody G protein-coupled receptor 9 antibody GPR9 antibody Interferon-inducible protein 10 receptor antibody IP-10 receptor antibody IP10 antibody IP10 R antibody IP10 receptor antibody IP10-R antibody IP10R antibody Mig R antibody Mig receptor antibody Mig-R antibody MIGR antibody |
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| Molecular Weight(MW) | 40.7kd |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P49682 |
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Application
Western blot analysis of CD183 against human CD183 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD183 against HEK293 (1) and CD183 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD183 against Hela (1) and L-02 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-CD183 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-60, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using anti-CD183 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-60, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD183 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-60, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Application
Flow cytometric analysis of CD183 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-60, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela and L-02 cell lysate, Jurkat cells, Ramos cells , cervical cancer tissues, esophageal cancer tissues |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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