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| Product Name | KIR3DL1 [6D9F6] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | AMB11 antibody CD158 antigen-like family member E antibody CD158e antibody CD158e antigen antibody CD158E1 antibody CD158E1/2 antibody CD158E2 antibody CL11 antibody CL2 antibody HLA-BW4-specific inhibitory NK cell receptor antibody KI3L1_HUMAN antibody killer cell immunoglobulin like receptor antibody Killer cell immunoglobulin like receptor three domains , short cytoplasmic tail, 1 antibody Killer cell immunoglobulin like receptor three domains long cytoplasmic tail 1 antibody Killer cell immunoglobulin-like receptor 3DL1 antibody KIR antibody KIR antigen 3DL1 antibody KIR G1 antibody Kir3dl1 antibody KIR3DS1 antibody Kirl1 antibody Kirl2 antibody Krl1 antibody MGC119726 antibody MGC119728 antibody MGC126589 antibody MGC126591 antibody MHC class I NK cell receptor antibody Natural killer associated transcript 3 antibody Natural killer cell inhibitory receptor antibody Natural killer-associated transcript 3 antibody NK receptor antibody NK-associated transcript 10 antibody NK-associated transcript 3 antibody NK-associated transcript 3delIg1 antibody NKAT-3 antibody NKAT10 antibody NKAT3 antibody NKB1 antibody NKB1B antibody p70 killer cell inhibitory receptor antibody p70 natural killer cell receptor clones CL 2/CL 11 antibody p70 natural killer cell receptor clones CL-2/CL-11 antibody p70 NK receptor CL-2/CL-11 antibody |
| Product description | Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. |
| Immunogen | Purified recombinant fragment of human KIR3DL1 (AA: extra 22-340) expressed in Hek293 cells. |
| Clonality | Monoclonal |
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| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | HumanRatMonkey |
| Concentration | 1mg/mL |
| Alternative Names | AMB11 antibody CD158 antigen-like family member E antibody CD158e antibody CD158e antigen antibody CD158E1 antibody CD158E1/2 antibody CD158E2 antibody CL11 antibody CL2 antibody HLA-BW4-specific inhibitory NK cell receptor antibody KI3L1_HUMAN antibody killer cell immunoglobulin like receptor antibody Killer cell immunoglobulin like receptor three domains short cytoplasmic tail 1 antibody Killer cell immunoglobulin like receptor three domains long cytoplasmic tail 1 antibody Killer cell immunoglobulin-like receptor 3DL1 antibody KIR antibody KIR antigen 3DL1 antibody KIR G1 antibody Kir3dl1 antibody KIR3DS1 antibody Kirl1 antibody Kirl2 antibody Krl1 antibody MGC119726 antibody MGC119728 antibody MGC126589 antibody MGC126591 antibody MHC class I NK cell receptor antibody Natural killer associated transcript 3 antibody Natural killer cell inhibitory receptor antibody Natural killer-associated transcript 3 antibody NK receptor antibody NK-associated transcript 10 antibody NK-associated transcript 3 antibody NK-associated transcript 3delIg1 antibody NKAT-3 antibody NKAT10 antibody NKAT3 antibody NKB1 antibody NKB1B antibody p70 killer cell inhibitory receptor antibody p70 natural killer cell receptor clones CL 2/CL 11 antibody p70 natural killer cell receptor clones CL-2/CL-11 antibody p70 NK receptor CL-2/CL-11 antibody |
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| Molecular Weight(MW) | 49kd |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P43629 |
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Application
Western blot analysis of KIR3DL1 against human KIR3DL1 (AA: extra 22-340) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of KIR3DL1 against A431 (1), Raji (2), SPC-A-1 (3), K562 (4), HEK293 (5), U937 (6), C6 (7), and COS7 (8) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-KIR3DL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-62, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | A431, Raji, SPC-A-1, K562, HEK293, U937, C6, and COS7 cell lysate, cervical cancer tissues |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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