CD329 antibody CDw329 antibody FOAP 9 antibody OB binding protein like antibody OBBP LIKE antibody Protein FOAP 9 antibody Protein FOAP-9 antibody sialic acid binding Ig like lectin 9 antibody Sialic acid-binding Ig-like lectin 9 antibody Sialic acid-binding immunoglobulin like lectin 9 antibody SIGL9_HUMAN antibody Siglec 9 antibody Siglec-9 antibody SIGLEC9 antibody
Product description
CD329 is also known as Sialic acid-binding Ig-like lectin 9 (Siglec-9), formerly known as Siglec-Y, which is expressed on peripheral blood monocytes, granulocytes and NK cells. Among its related pathways are Class I MHC mediated antigen processing and presentation and Innate Immune System. GO annotations related to this gene include carbohydrate binding. Siglecs (sialic acid/immunolgobulin/lectin) are a family of I-type lectins binding to sialic acids on the cell surface. They are a family of carbohydrate-binding proteins within the immunoglobulin superfamily. Siglecs are all integral membrane proteins with extracellular domains consisting of unusual N-termial V-set Ig domains, followed by variable numbers of C2-set Ig domains. It is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It also binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Immunogen
Purified recombinant fragment of human CD329 (AA: extra 18–348) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
CD329 antibody CDw329 antibody FOAP 9 antibody OB binding protein like antibody OBBP LIKE antibody Protein FOAP 9 antibody Protein FOAP-9 antibody sialic acid binding Ig like lectin 9 antibody Sialic acid-binding Ig-like lectin 9 antibody Sialic acid-binding immunoglobulin like lectin 9 antibody SIGL9_HUMAN antibody Siglec 9 antibody Siglec-9 antibody SIGLEC9 antibody
Western blot analysis of CD329 against human CD329 (AA: extra 18–348) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-18 against HEK293 (1) and CD329 (AA: extra 18–348)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD329 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-18, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
Jurkat cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
