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| Product Name | TRK fused gene [SD082-0] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ36137 antibody HMSNP antibody OTTHUMP00000214045 antibody OTTHUMP00000214046 antibody OTTHUMP00000214047 antibody OTTHUMP00000214048 antibody Protein TFG antibody SPG57 antibody TF6 antibody TFG antibody TFG_HUMAN antibody TRK fused antibody TRK fused gene antibody TRK fused gene protein antibody TRK-fused gene protein antibody TRKT3 antibody TRKT3 oncogene antibody |
| Product description | Oncogenic rearrangements of the NTRK1 gene, which encodes the Trk A protein, are frequently detected in thyroid carcinomas. Such rearrangements fuse the NTRK1 tyrosine kinase domain to 5'-end sequences of different genes. TRK-T3 contains 1,412 nucleotides of NTRK1 preceded by 598 nucleotides belonging to TFG (TRK-fused gene), a ubiquitously expressed gene located on chromosome 3. The TRK-T3 protein within the TFG region contains a coiled-coil motif that gives the oncoprotein the capability to form complexes. The cytoplasmic TRK-T3 protein binds to and phosphorylates the Shc and SNT1/FRS2 adaptor proteins, both of which are involved in coupling the receptor tyrosine kinase to the mitogen-activated protein kinase pathway by recruiting Grb2/SOS. SHP-1 also interacts with and down-regulates TRK-T3. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ36137 antibody HMSNP antibody OTTHUMP00000214045 antibody OTTHUMP00000214046 antibody OTTHUMP00000214047 antibody OTTHUMP00000214048 antibody Protein TFG antibody SPG57 antibody TF6 antibody TFG antibody TFG_HUMAN antibody TRK fused antibody TRK fused gene antibody TRK fused gene protein antibody TRK-fused gene protein antibody TRKT3 antibody TRKT3 oncogene antibody |
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| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela
Application
Fig2: ICC staining TRK fused gene in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TRK fused gene in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TRK fused gene in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.| Positive Control | SKOV-3, A549, Hela, 293T, human lung cancer tissue, human tonsil tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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