| 存储条件 |
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| Product Name | TrkA+B+C [JJ084-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BDNF/NT-3 growth factors receptor antibody gp140trk antibody GP145-TrkB antibody GP145-TrkC antibody High affinity nerve growth factor receptor antibody MTC antibody Neurotrophic tyrosine kinase receptor type 1 antibody Neurotrophic tyrosine kinase receptor type 2 antibody Neurotrophic tyrosine kinase receptor type 3 antibody NT-3 growth factor receptor antibody NTRK1 antibody NTRK2 antibody NTRK3 antibody p140-TrkA antibody TRK antibody Trk-A antibody Trk-B antibody Trk-C antibody TRK1-transforming tyrosine kinase protein antibody TRKA antibody TRKB antibody TrkB tyrosine kinase antibody TRKC antibody TrkC tyrosine kinase antibody Tropomyosin-related kinase A antibody Tropomyosin-related kinase B antibody Tyrosine kinase receptor A antibody Tyrosine kinase receptor antibody |
| Product description | The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system. Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas. TrkB is overexpressed in tumors such as neuroblastoma, prostate adenocarcinoma and pancreatic ductal adenocarcinoma. In neuroblastomas overexpression of TrkB correlates with unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms’ tumors and this isoform may act as a dominant-negative to TrkB signaling. Altered TrkC expression and corresponding gene mutations are seen in various forms of cancer, with increased expression a positive prognostic indicator in patients with medulloblastoma. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BDNF/NT-3 growth factors receptor antibody gp140trk antibody GP145-TrkB antibody GP145-TrkC antibody High affinity nerve growth factor receptor antibody MTC antibody Neurotrophic tyrosine kinase receptor type 1 antibody Neurotrophic tyrosine kinase receptor type 2 antibody Neurotrophic tyrosine kinase receptor type 3 antibody NT-3 growth factor receptor antibody NTRK1 antibody NTRK2 antibody NTRK3 antibody p140-TrkA antibody TRK antibody Trk-A antibody Trk-B antibody Trk-C antibody TRK1-transforming tyrosine kinase protein antibody TRKA antibody TRKB antibody TrkB tyrosine kinase antibody TRKC antibody TrkC tyrosine kinase antibody Tropomyosin-related kinase A antibody Tropomyosin-related kinase B antibody Tyrosine kinase receptor A antibody Tyrosine kinase receptor antibody |
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| Molecular Weight(MW) | 140 kDa |
| Cellular Localization | Membrane, Endosome membrane. |
Application
Fig1: Western blot analysis of TrkA+B+C on different lysates using anti-TrkA+B+C antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse brain
Application
Fig2: ICC staining TrkA+B+C in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TrkA+B+C in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-TrkA+B+C antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TrkA+B+C antibody. Counter stained with hematoxylin.| Positive Control | N2A, SH-SY-5Y, rat brain tissue, mouse liver tissue, mouse brain tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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