| 存储条件 |
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| Product Name | Cytokeratin 6 [SN71-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK 6A antibody CK 6B antibody CK 6C antibody CK 6D antibody CK 6E antibody CK-6C antibody CK-6E antibody Cytokeratin 6a antibody Cytokeratin 6B antibody Cytokeratin 6C antibody Cytokeratin 6D antibody Cytokeratin 6E antibody Cytokeratin-6C antibody Cytokeratin-6E antibody K2C6C_HUMAN antibody K6a keratin antibody K6b keratin antibody K6C antibody K6c keratin antibody K6d keratin antibody K6e keratin antibody Keratin antibody Keratin K6h antibody Keratin type II cytoskeletal 6A antibody Keratin type II cytoskeletal 6B antibody Keratin type II cytoskeletal 6C antibody Keratin type II cytoskeletal 6D antibody Keratin type II cytoskeletal 6E antibody Keratin-6C antibody KRT6A antibody KRT6B antibody KRT6C antibody KRT6D antibody KRT6E antibody type II cytoskeletal 6C antibody Type-II keratin Kb12 antibody |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CK 6A antibody CK 6B antibody CK 6C antibody CK 6D antibody CK 6E antibody CK-6C antibody CK-6E antibody Cytokeratin 6a antibody Cytokeratin 6B antibody Cytokeratin 6C antibody Cytokeratin 6D antibody Cytokeratin 6E antibody Cytokeratin-6C antibody Cytokeratin-6E antibody K2C6C_HUMAN antibody K6a keratin antibody K6b keratin antibody K6C antibody K6c keratin antibody K6d keratin antibody K6e keratin antibody Keratin antibody Keratin K6h antibody Keratin type II cytoskeletal 6A antibody Keratin type II cytoskeletal 6B antibody Keratin type II cytoskeletal 6C antibody Keratin type II cytoskeletal 6D antibody Keratin type II cytoskeletal 6E antibody Keratin-6C antibody KRT6A antibody KRT6B antibody KRT6C antibody KRT6D antibody KRT6E antibody type II cytoskeletal 6C antibody Type-II keratin Kb12 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
Application
Fig1: Western blot analysis of Cytokeratin 6 on human skin lysates using anti-Cytokeratin 6 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cytokeratin 6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 6 antibody. Counter stained with hematoxylin.| Positive Control | A431, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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