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| Product Name | TMS1 [SN07-10] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis associated speck like protein containing a CARD antibody Apoptosis-associated speck-like protein containing a CARD antibody ASC antibody ASC_HUMAN antibody CARD 5 antibody CARD5 antibody Caspase recruitment domain containing protein 5 antibody Caspase recruitment domain protein 5 antibody Caspase recruitment domain-containing protein 5 antibody hASC antibody MGC10332 antibody PYCARD antibody PYD and CARD domain containing antibody PYD and CARD domain containing protein antibody PYD and CARD domain-containing protein antibody Target of methylation induced silencing 1 antibody Target of methylation-induced silencing 1 antibody TMS 1 antibody TMS antibody TMS1 antibody |
| Product description | The death domain (DD) superfamily of proteins share one or more of the following domains: the DD, DED (death-effector domain), CARD (caspase-recruitment domain) and PYD (Pyrin domain). Each of these domains is characterized by a canonical death domain fold, which consists of a bundle of five or six antiparallel α-helices. As their names suggest, these domains play prominent roles in programmed cell death. Caspase-associated recruitment domains (CARDs) mediate the interaction between adaptor proteins such as Apaf-1 and the proform of caspases (e.g., CASP9) participating in apoptosis. ASC (apoptosis-associated speck-like protein containing a CARD, also known as TMS1or PYCARD) is a member of the CARD-containing adaptor protein family. ASC is a 195 amino acid protein, containing a N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal CARD. This motif is characteristic of numerous proteins involved in apoptotic signaling. ASC2 (apoptosis-associated speck-like protein containing a CARD 2), also known as Pyrin-only protein 1 or PADD-only protein 1, is an 89 amino acid member of the DD superfamily that contains one Pyrin domain. Localized to the cytoplasm, ASC2 interacts with ASC to modulate NF-κB and pro-caspase-1 regulation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis associated speck like protein containing a CARD antibody Apoptosis-associated speck-like protein containing a CARD antibody ASC antibody ASC_HUMAN antibody CARD 5 antibody CARD5 antibody Caspase recruitment domain containing protein 5 antibody Caspase recruitment domain protein 5 antibody Caspase recruitment domain-containing protein 5 antibody hASC antibody MGC10332 antibody PYCARD antibody PYD and CARD domain containing antibody PYD and CARD domain containing protein antibody PYD and CARD domain-containing protein antibody Target of methylation induced silencing 1 antibody Target of methylation-induced silencing 1 antibody TMS 1 antibody TMS antibody TMS1 antibody |
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| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus. |
| SwissProt ID | Q9ULZ3 |
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Application
Fig1: ICC staining TMS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining TMS1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TMS1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of 293 cells with TMS1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | A549, Hela, HepG2, 293. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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