| 存储条件 |
|---|
| Product Name | CCR2 [SN707] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C C chemokine receptor type 2 antibody C C CKR 2 antibody C-C chemokine receptor type 2 antibody C-C CKR-2 antibody CC chemokine receptor type 2 antibody CC CKR 2 antibody CC-CKR-2 antibody CCCKR2 antibody CCR 2 antibody CCR-2 antibody CCR1L antibody CCR2 antibody CCR2_HUMAN antibody CCR2A antibody CCR2B antibody CCR5L antibody CD192 antibody CD192 antigen antibody Chemokine C C motif receptor 2 antibody Chemokine CC Motif Receptor 2 antibody CKR 2 antibody CKR2 antibody CKR2A antibody CKR2B antibody CMKBR2 antibody MCP 1 R antibody MCP-1-R antibody MCP1 RECEPTOR antibody MCP1R antibody Monocyte chemoattractant protein 1 receptor antibody Monocyte Chemotactic Protein 1 Receptor antibody Monocyte Chemotactic Protein 1 Receptor antibody |
| Product description | Key functional receptor for CCL2 but can also bind CCL7 and CCL12. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (Probable). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation. Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. Mediates the recruitment of macrophages and monocytes to the injury site following brain injury. |
| Immunogen | Recombinant protein within N-terminal Human CCR2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:500-1:1,000 IHC:1:100-1:200: FC:1:50-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | C C chemokine receptor type 2 antibody C C CKR 2 antibody C-C chemokine receptor type 2 antibody C-C CKR-2 antibody CC chemokine receptor type 2 antibody CC CKR 2 antibody CC-CKR-2 antibody CCCKR2 antibody CCR 2 antibody CCR-2 antibody CCR1L antibody CCR2 antibody CCR2_HUMAN antibody CCR2A antibody CCR2B antibody CCR5L antibody CD192 antibody CD192 antigen antibody Chemokine C C motif receptor 2 antibody Chemokine CC Motif Receptor 2 antibody CKR 2 antibody CKR2 antibody CKR2A antibody CKR2B antibody CMKBR2 antibody MCP 1 R antibody MCP-1-R antibody MCP1 RECEPTOR antibody MCP1R antibody Monocyte chemoattractant protein 1 receptor antibody Monocyte Chemotactic Protein 1 Receptor antibody Monocyte Chemotactic Protein 1 Receptor antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: THP-1 cell lysate
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Flow cytometric analysis of CCR2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Mouse spleen tissue lysate, THP-1 cell lysate, human tonsil tissue, human breast carcinoma tissue, K562 cell. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:200: FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
