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| Product Name | NBN [A2-E4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AT V1 antibody AT V2 antibody ATV antibody Cell cycle regulatory protein p95 antibody FLJ10155 antibody MGC87362 antibody Nbn antibody NBN_HUMAN antibody NBS 1 antibody NBS antibody NBS1 antibody Nibrin antibody Nijmegen breakage syndrome 1 (nibrin) antibody Nijmegen breakage syndrome antibody Nijmegen breakage syndrome protein 1 antibody p95 antibody p95 protein of the MRE11/RAD50 complex antibody |
| Product description | Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | AT V1 antibody AT V2 antibody ATV antibody Cell cycle regulatory protein p95 antibody FLJ10155 antibody MGC87362 antibody Nbn antibody NBN_HUMAN antibody NBS 1 antibody NBS antibody NBS1 antibody Nibrin antibody Nijmegen breakage syndrome 1 (nibrin) antibody Nijmegen breakage syndrome antibody Nijmegen breakage syndrome protein 1 antibody p95 antibody p95 protein of the MRE11/RAD50 complex antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa |
| Cellular Localization | Nucleus. Nucleus, PML body. |
Application
Fig1: Western blot analysis of p95 NBS1 on human p95 NBS1 recombinant protein using anti- p95 NBS1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of p95 NBS1 on HEK293 (1) and p95 NBS1 -hIgGFc transfected HEK293 (2) cell lysate using anti- p95 NBS1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of p95 NBS1 on different cell lysate using anti- p95 NBS1 antibody at 1/1,000 dilution. Positive control： Line1: A549 Line2: Jurkat Line3: PC-12
Application
Fig4: ICC staining p95 NBS1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- p95 NBS1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- p95 NBS1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with p95 NBS1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human p95 NBS1 recombinant protein, p95 NBS1 -hIgGFc transfected HEK293 cell lysate, A549, Jurkat, PC-12 cell lysate, human cervical cancer tissue, human rectum cancer tissue, Hela. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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