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| Product Name | SIRT4 [D7-A12] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC130046 antibody MGC130047 antibody MGC57437 antibody NAD dependent deacetylase sirtuin 4 antibody NAD dependent protein deacetylase sirtuin 4 antibody NAD-dependent ADP-ribosyltransferase sirtuin-4 antibody NAD-dependent protein deacetylase sirtuin-4 antibody NAD-dependent protein lipoamidase sirtuin-4, mitochondrial antibody Regulatory protein SIR2 homolog 4 antibody Silent mating type information regulation 2 homolog antibody Sir 2 like 4 antibody SIR 2 like protein 4 antibody Sir2 like 4 antibody SIR2 like protein 4 antibody SIR2-like protein 4 antibody SIR2L 4 antibody SIR2L4 antibody SIR4_HUMAN antibody SIRT 4 antibody Sirt4 antibody Sirtuin (silent mating type information regulation 2 homolog) 4 antibody Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody Sirtuin 4 antibody Sirtuin type 4 antibody Sirtuin-4 antibody Sirtuin4 antibody |
| Product description | Sirtuins (SIRT1-7) are human homologs of the yeast Sir2 (silent information regulator-2) protein and are divided into four main classes: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. In S. cerevisiae, Sir2 deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA (ribosomal DNA) and silent mating-type loci. The human SIRT proteins are NAD-dependent deacetylases that act as intracellular regulators and are thought to have ribosyltransferase activity. SIRT4 (SIR2-like protein 4), also known as SIR2L4 or sirtuin 4, belongs to the class II family of sirtuins and localizes to the mitochondrial matrix. Expressed throughout the body, SIRT4 interacts with insulin-degrading enzymes and, through its ADP-ribosyltransferase activity, functions to negatively regulate insulin secretion from pancreatic β cells. SIRT4 contains one deacetylase sirtuin-type domain and can bind zinc as a catalytic cofactor. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | MGC130046 antibody MGC130047 antibody MGC57437 antibody NAD dependent deacetylase sirtuin 4 antibody NAD dependent protein deacetylase sirtuin 4 antibody NAD-dependent ADP-ribosyltransferase sirtuin-4 antibody NAD-dependent protein deacetylase sirtuin-4 antibody NAD-dependent protein lipoamidase sirtuin-4 mitochondrial antibody Regulatory protein SIR2 homolog 4 antibody Silent mating type information regulation 2 homolog antibody Sir 2 like 4 antibody SIR 2 like protein 4 antibody Sir2 like 4 antibody SIR2 like protein 4 antibody SIR2-like protein 4 antibody SIR2L 4 antibody SIR2L4 antibody SIR4_HUMAN antibody SIRT 4 antibody Sirt4 antibody Sirtuin (silent mating type information regulation 2 homolog) 4 antibody Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody Sirtuin 4 antibody Sirtuin type 4 antibody Sirtuin-4 antibody Sirtuin4 antibody |
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| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Mitochondrion matrix |
| SwissProt ID | Q9Y6E7 |
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Application
Fig1: Western blot analysis of SIRT4 on human SIRT4 recombinant protein using anti-SIRT4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SIRT4 on HEK293 (1) and SIRT4-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT4 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining SIRT4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with SIRT4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SIRT4 recombinant protein, SIRT4-hIgGFc transfected HEK293, Hela. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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