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| Product Name | DSG3 [11G-2C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 130 kD pemphigus vulgaris antigen antibody 130 kDa pemphigus vulgaris antigen antibody Balding antibody Cadherin family member 6 antibody CDHF 6 antibody CDHF6 antibody Desmoglein 3 antibody Desmoglein 3 precursor antibody Desmoglein-3 antibody Desmoglein3 antibody DKFZp686P23184 antibody DSG 3 antibody DSG3 antibody DSG3_HUMAN antibody Pemphigus vulgaris antigen antibody PVA antibody |
| Product description | Pemphigus is an autoimmune disease of skin adhesion associated with auto-antibodies against a number of keratinocyte antigens, such as the adhesion molecules desmoglein (dsg) 1 and 3 and acetylcholine receptors. Desmogleins, type I membrane proteins, are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions. Desmogleins belong to the cadherin family and consist of dsg1, dsg2 and dsg3. Calcium binds to the putative calcium binding sites at the extracellular N-terminal domain, which has cadherin-like repeats. Unlike normal human keratinocytes, the squamous cell carcinoma cells exhibit diminished or un-usual expression of dsg3 and dsg1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively. Several carcinoma cell lines constantly express dsg2 and dsg3 mRNA, whereas cultured normal human keratinocytes always express dsg1 and dsg3 mRNA, with or without dsg2 mRNA. This expression pattern indicates that desmoglein isoforms exhibit abnormal expression and may be related to tumor cell kinetics, such as cell invasion and metastasis. dsg2 is the fundamental dsg common to all desmosome-possessing tissues and is the largest desmoglein in the family. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:200-1:1,000 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 130 kD pemphigus vulgaris antigen antibody 130 kDa pemphigus vulgaris antigen antibody Balding antibody Cadherin family member 6 antibody CDHF 6 antibody CDHF6 antibody Desmoglein 3 antibody Desmoglein 3 precursor antibody Desmoglein-3 antibody Desmoglein3 antibody DKFZp686P23184 antibody DSG 3 antibody DSG3 antibody DSG3_HUMAN antibody Pemphigus vulgaris antigen antibody PVA antibody |
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| Molecular Weight(MW) | 108 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P32926 |
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Application
Fig1: Western blot analysis of DSG3 on human DSG3 recombinant protein using anti-DSG3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DSG3 on HEK293 (1) and DSG3-hIgGFc transfected HEK293 (2) cell lysate using anti-DSG3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DSG3 on A431 cell lysate using anti-DSG3 antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DSG3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal tissue using anti-DSG3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A431 cells with DSG3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DSG3 recombinant protein, DSG3-hIgGFc transfected HEK293 cell lysate, A431, human esophageal cancer tissue, human esophageal tissue. |
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| Application Notes | WB:1:500-1:2,000 IHC:1:200-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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