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| Product Name | VTN [G11-E8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Complement S Protein antibody Epibolin antibody S Protein antibody S-protein antibody Serum Spreading Factor antibody Serum-spreading factor antibody Somatomedin B antibody Somatomedin-B antibody V75 antibody Vitronectin V10 subunit antibody Vitronectin V65 subunit antibody VN antibody VNT antibody VTN antibody VTNC_HUMAN antibody |
| Product description | Fibronectin and Vitronectin are extracellular matrix glycoproteins that are present on most cell surfaces, in extracellular fluids, and in plasma. Both Fibronectin and Vitronectin have been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Vitronectin contains an RGD (Arg-Gly-Asp acid) sequence that is present in many cell adhesion ligands. The RGD sequence has been shown to be essential for cell adhesion. Increased expression of Vitronectin, integrins and plasminogen activators has been observed in migrating cells during wound healing. Vitronectin has been shown to enhance smooth cell migration, and PAI-1 has been shown to bind to Vitronectin with high affinity, resulting in the blocking of smooth cell migration. Glycosaminoglycans, proteins involved in the anchoring of vitronectin to the extracellular matrix, have been shown to stimulate the cleavage of vitronectin by plasmin. This cleavage reduces the affinity of vitronectin for PAI-1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Complement S Protein antibody Epibolin antibody S Protein antibody S-protein antibody Serum Spreading Factor antibody Serum-spreading factor antibody Somatomedin B antibody Somatomedin-B antibody V75 antibody Vitronectin V10 subunit antibody Vitronectin V65 subunit antibody VN antibody VNT antibody VTN antibody VTNC_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Secreted, extracellular space |
| SwissProt ID | P04004 |
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Application
Fig1: Western blot analysis of VTN on human VTN recombinant protein using anti-VTN antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of VTN on HEK293 (1) and VTN-hIgGFc transfected HEK293 (2) cell lysate using anti-VTN antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-VTN antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-VTN antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with VTN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human VTN recombinant protein, VTN-hIgGFc transfected HEK293 cell lysate, human cervical cancer tissue, human liver cancer tissue, Hela. |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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