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Cytokeratin 14 [SC65-06]

Cytokeratin 14 [SC65-06]

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基础信息
存储条件
产品详情
Product Profile
Product NameCytokeratin 14 [SC65-06]
Antibody TypePrimary Antibodies
Antigen AliasCK 14 antibody CK-14 antibody ck14 antibody Cytokeratin 14 antibody Cytokeratin-14 antibody Cytokeratin14 antibody Dowling Meara antibody EBS3 antibody EBS4 antibody Epidermolysis bullosa simplex antibody K14 antibody K1C14_HUMAN antibody Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody Keratin 14 antibody Keratin antibody Keratin type I cytoskeletal 14 antibody Keratin, type I cytoskeletal 14 antibody Keratin-14 antibody Keratin14 antibody Koebner antibody Krt 14 antibody Krt14 antibody NFJ antibody OTTHUMP00000164624 antibody type I cytoskeletal 14 antibody
Product descriptionarkers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. The gene encoding human Cytokeratin 14 maps to chromosome 17q12-21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.
Immunogenrecombinant protein
Key Feature
ClonalityMonoclonal
IsotypeIgG
Host SpeciesRecombinant rabbit
Tested ApplicationsWBICC/IFIHC

WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500
Species ReactivityHumanMouseRat
Concentration1mg/ml
Target Information
Alternative NamesCK 14 antibody CK-14 antibody ck14 antibody Cytokeratin 14 antibody Cytokeratin-14 antibody Cytokeratin14 antibody Dowling Meara antibody EBS3 antibody EBS4 antibody Epidermolysis bullosa simplex antibody K14 antibody K1C14_HUMAN antibody Keratin 14 (epidermolysis bullosa simplex
Dowling-Meara
Koebner) antibody Keratin 14 antibody Keratin antibody Keratin type I cytoskeletal 14 antibody Keratin
type I cytoskeletal 14 antibody Keratin-14 antibody Keratin14 antibody Koebner antibody Krt 14 antibody Krt14 antibody NFJ antibody OTTHUMP00000164624 antibody type I cytoskeletal 14 antibody
Molecular Weight(MW)53 kDa
Cellular LocalizationCytoplasm, Nucleus.
Database Links
SwissProt IDP02533Q61781Q6IFV1
Application

Application

Fig1: Western blot analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin

Application

Fig2: ICC staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig3: ICC staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig4: ICC staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig5: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.

Application

Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.

Application

Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.
Positive ControlA431, B16-F1, SW480, HepG2, human breast carcinoma tissue, mouse prostate tissue, mouse skin tissue, mouse prostate tissue.
Application NotesWB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500
Additional Information
FormLiquid
Storage InstructionsStore at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.


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