| 存储条件 |
|---|
| Product Name | Met(C-Met) [SJ19-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AUTS9 antibody c met antibody D249 antibody Hepatocyte growth factor receptor antibody HGF antibody HGF receptor antibody HGF/SF receptor antibody HGFR antibody MET antibody Met proto oncogene tyrosine kinase antibody MET proto oncogene receptor tyrosine kinase antibody Met proto-oncogene (hepatocyte growth factor receptor) antibody Met proto-oncogene antibody Met protooncogene antibody MET_HUMAN antibody Oncogene MET antibody Par4 antibody Proto-oncogene c-Met antibody RCCP2 antibody Scatter factor receptor antibody SF receptor antibody Tyrosine-protein kinase Met antibody |
|---|---|
| Molecular Weight(MW) | 155 kDa |
| SwissProt ID | P08581 |
|---|
Application Fig1: Western blot analysis of Met on different lysates using anti-Met antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2
Application Fig2: ICC staining Met in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Met antibody. Counter stained with hematoxylin.
Application Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Met antibody. Counter stained with hematoxylin.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Met antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Met antibody. Counter stained with hematoxylin.
Application Fig7: Flow cytometric analysis of Hela cells with Met antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | Hela, HepG2, human tonsil tissue, human lung cancer tissue, human liver cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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