| 存储条件 |
|---|
| Product Name | PI 3 Kinase catalytic subunit alpha [SJ0186] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IP |
| WB:1:500-1:1,000 ICC:1:50-1:200 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody caPI3K antibody CLOVE antibody CWS5 antibody MCAP antibody MCM antibody MCMTC antibody MGC142161 antibody MGC142163 antibody p110 alpha antibody p110alpha antibody Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody Phosphatidylinositol-4 antibody Phosphoinositide 3 kinase catalytic alpha polypeptide antibody PI3 kinase p110 subunit alpha antibody PI3-kinase subunit alpha antibody PI3K antibody PI3K-alpha antibody PI3KC A antibody PIK3C A antibody Pik3ca antibody PK3CA antibody PK3CA_HUMAN antibody PtdIns 3 kinase p110 antibody PtdIns-3-kinase subunit alpha antibody PtdIns-3-kinase subunit p110-alpha antibody Serine/threonine protein kinase PIK3CA antibody |
|---|---|
| Molecular Weight(MW) | 124 kDa |
| Cellular Localization | Cytosol. |
Application
Fig1: ICC staining PI 3 kinase p110 alpha in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining PI 3 kinase p110 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PI 3 kinase p110 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | MCF-7, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
