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| Product Name | Bcl-XL [SZ3-03] |
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| Antibody Type | Primary Antibodies |
| Product description | The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18) bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A second protein, designated Bcl-associated X protein (Bax) p21, has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis regulator Bcl X antibody Apoptosis regulator Bcl-X antibody Apoptosis regulator BclX antibody B cell lymphoma 2 like antibody B2CL1_HUMAN antibody Bcl 2 like 1 protein antibody Bcl X antibody Bcl xL antibody BCL XL/S antibody Bcl xS antibody Bcl-2-like protein 1 antibody Bcl2 Like 1 antibody Bcl2 related gene antibody Bcl2-L-1 antibody BCL2L antibody Bcl2l1 antibody BCLX antibody BclXL antibody BclXs antibody DKFZp781P2092 antibody PPP1R52 antibody Protein phosphatase 1 regulatory subunit 52 antibody |
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| Molecular Weight(MW) | 26 kDa |
Application Fig1: ICC staining Bcl-XL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig2: ICC staining Bcl-XL in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining Bcl-XL in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.
Application Fig7: Flow cytometric analysis of Hela cells with Bcl-XL antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | MCF-7, Hela, HepG2, human colon cancer tissue, human breast carcinoma tissue, human kidney tissue. |
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| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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