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| Product Name | IMP-3 [C2-E4] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Cancer/testis antigen 98 antibody CT98 antibody DKFZp686F1078 antibody hKOC antibody IF2B3_HUMAN antibody IGF II mRNA binding protein 3 antibody IGF-II mRNA-binding protein 3 antibody IGF2 mRNA binding protein 3 antibody IGF2 mRNA-binding protein 3 antibody IGF2BP3 antibody IMP 3 antibody IMP-3 antibody Insulin like growth factor 2 mRNA binding protein 3 antibody Insulin-like growth factor 2 mRNA-binding protein 3 antibody KH domain containing protein overexpressed in cancer antibody KH domain-containing protein overexpressed in cancer antibody KOC 1 antibody KOC1 antibody VICKZ 3 antibody VICKZ family member 3 antibody VICKZ3 antibody |
| Product description | Insulin like growth factor 2 mRNA binding proteins (IGF2BPs) bind RNA and influence RNA synthesis and metabolism. IGF2BP1, also known as coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein (CRD-BP), IMP1 or VICKZ1; IGF2BP2 (IMP2, VICKZ2, p62); and IGF2BP2 (IMP3, KOC1, VICKZ3) contain a unique combination of RNA recognition motifs and four hnRNP K homology domains. IGF2BP1 is abundant in embryonal tissues and is expressed in 81% of colon cancers, 73% of sarcomas and 58.5% of breast cancers. It recognizes c-Myc, IGF-II and t mRNAs, and H19 RNA, and plays a major role in proliferation of K-562 cells by an IGF-II-dependent mechanism. IGF2BP2 binds the 5' UTR of IGF-II mRNA and influences tumor cell growth, in which IGF2BP2 is associated with apoptosis induced by tretinoin. IGF2BP3 knockdown by RNA interference decreases levels of IGF-II protein without affecting IGF-II, c-Myc, or β Actin mRNA and H19 RNA levels. IGF2BP3 is a marker for carcinomas and high-grade dysplastic lesions of pancreatic ductal epithelium. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:1,000-1:2,000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100: | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Cancer/testis antigen 98 antibody CT98 antibody DKFZp686F1078 antibody hKOC antibody IF2B3_HUMAN antibody IGF II mRNA binding protein 3 antibody IGF-II mRNA-binding protein 3 antibody IGF2 mRNA binding protein 3 antibody IGF2 mRNA-binding protein 3 antibody IGF2BP3 antibody IMP 3 antibody IMP-3 antibody Insulin like growth factor 2 mRNA binding protein 3 antibody Insulin-like growth factor 2 mRNA-binding protein 3 antibody KH domain containing protein overexpressed in cancer antibody KH domain-containing protein overexpressed in cancer antibody KOC 1 antibody KOC1 antibody VICKZ 3 antibody VICKZ family member 3 antibody VICKZ3 antibody |
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| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of IMP3 on Hela lysate using anti-IMP3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of IMP3 on 293T lysate using anti-IMP3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining IMP3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IMP3 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IMP3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IMP3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP3 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of HUVEC cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Hela, 293T, SH-SY5Y, rat brain tissue, human liver cancer tissue, human placenta tissue, mouse testis tissue, HUVEC. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100: |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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