| 存储条件 |
|---|
| Product Name | Cytokeratin 13 [SN71-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 13 belongs to the intermediate filament family and is a heterotetramer of two type I acidic and two type II basic keratins. It is generally associated with cytokeratin 4. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder which predominantly affects noncornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC,IHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | Human,Mouse,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 47 kDa cytokeratin antibody CK-13 antibody CK13 antibody Cytokeratin 13 antibody Cytokeratin-13 antibody K13 antibody K1C13_HUMAN antibody Ka13 antibody Keratin 13 antibody Keratin antibody keratin type I cytoskeletal 13 antibody Keratin-13 antibody Krt-1.13 antibody Krt1-13 antibody KRT13 antibody MGC161462 antibody MGC3781 antibody type I cytoskeletal 13 antibody Type I keratin Ka13 antibody WSN2 antibody |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Extracellular exosome, intermediate filament cytoskeleton, keratin filament, nucleus. |
Application
Fig1: Western blot analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cytokeratin 13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cytokeratin 13 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cytokeratin 13 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.
Application
Fig7:Western blot analysis of Cytokeratin 13 hybrid fish (crucian-carp) brain tissue lysate using anti-Cytokeratin 13 antibody at 1/500 dilution.| Positive Control | A549, MCF-7, Hela, human breast carcinoma tissue, human lung tissue, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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