For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
Application
Dilution
Western Blotting
1:2000
Immunoprecipitation
1:100
Immunofluorescence (Immunocytochemistry)
1:800 - 1:1600
Flow Cytometry (Fixed/Permeabilized)
1:800 - 1:3200
Chromatin IP
1:100
Chromatin IP-seq
1:100
CUT&RUN
1:50
CUT&Tag
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #45772.
Specificity / Sensitivity
Ubiquityl-Histone H2A (Lys119) (D27C4) Rabbit Monoclonal Antibody recognizes endogenous levels of histone H2A protein only when ubiquitinated at Lys119. This antibody does not cross-react with other ubiquitinated proteins or free ubiquitin.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human histone H2A protein in which Lys119 is mono-ubiquitinated.
