Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Specificity / Sensitivity
SMAD3 (C67H9) Rabbit mAb detects endogenous levels of total SMAD3 protein. No cross reactivity was detected with other family members.
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology:
Xenopus, Zebrafish, Bovine
