For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:2000
Fluorescent Western
1:2000
Simple Western™
1:10 - 1:50
Immunoprecipitation
1:100
IHC Leica Bond
1:100 - 1:400
Immunohistochemistry (Paraffin)
1:100 - 1:400
Immunofluorescence (Immunocytochemistry)
1:100 - 1:200
Flow Cytometry (Fixed/Permeabilized)
1:100 - 1:400
Chromatin IP
1:100
Chromatin IP-seq
1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #73533.
Specificity / Sensitivity
Phospho-Stat3 (Tyr705) (D3A7) Rabbit Monoclonal Antibody detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology:
Hamster, Bovine, Pig, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.
