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Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 488 Conjugate)

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包装规格 交货周期 质量标准 目录价 会员专享价 数量
100 µl 现货2-3天 原装正品
- +
基础信息
存储条件 +4℃
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Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with Myc-tagged protein.

Product Usage Information

ApplicationDilution
Immunofluorescence (Immunocytochemistry)1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Myc-Tag (9B11) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody conjugate recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody conjugate detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody conjugate may cross-react with c-myc protein.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.


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