For optimal ChIP and ChIP-seq results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:10 - 1:50
Immunoprecipitation
1:50
IHC Leica Bond
1:50 - 1:200
Immunohistochemistry (Paraffin)
1:50 - 1:200
Immunofluorescence (Frozen)
1:50 - 1:100
Immunofluorescence (Immunocytochemistry)
1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)
1:50 - 1:100
Chromatin IP
1:25
Chromatin IP-seq
1:25
CUT&RUN
1:25
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #84441.
Specificity / Sensitivity
beta-Catenin (D10A8) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-catenin protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology:
Zebrafish, Bovine, Pig, Horse, Guinea Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.
