For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:10 - 1:50
Immunoprecipitation
1:50
IHC Leica Bond
1:100 - 1:400
Immunohistochemistry (Paraffin)
1:200 - 1:800
Immunofluorescence (Frozen)
1:50 - 1:200
Immunofluorescence (Immunocytochemistry)
1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized)
1:50 - 1:200
Chromatin IP
1:50
Chromatin IP-seq
1:50
CUT&RUN
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #53921.
Specificity / Sensitivity
YAP (D8H1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total YAP protein. Non-specific labeling of membrane within fixed frozen mouse small intestine may be observed by immunofluorescence.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Species predicted to react based on 100% sequence homology:
Bovine, Horse, Guinea Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human YAP protein. The epitope corresponds to a region surrounding Pro435 of human YAP isoform 1. This sequence region is 100% conserved among all known isoforms of human YAP protein.
