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YAP (D8H1X) XP ® Rabbit mAb

产品编号 : 14074S

英文名称 :

中文名称 : 抗体

品牌 : CST

包装规格	交货周期	质量标准	目录价	会员专享价	数量
100 µl	现货2-3天	原装正品	￥4990	登录后可见	- +

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基础信息产品详情相关文献

基础信息

存储条件	-20℃

产品详情

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
IHC Leica Bond	1:100 - 1:400
Immunohistochemistry (Paraffin)	1:200 - 1:800
Immunofluorescence (Frozen)	1:50 - 1:200
Immunofluorescence (Immunocytochemistry)	1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized)	1:50 - 1:200
Chromatin IP	1:50
Chromatin IP-seq	1:50
CUT&RUN	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #53921.

Specificity / Sensitivity

YAP (D8H1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total YAP protein. Non-specific labeling of membrane within fixed frozen mouse small intestine may be observed by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

Species predicted to react based on 100% sequence homology:

Bovine, Horse, Guinea Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human YAP protein. The epitope corresponds to a region surrounding Pro435 of human YAP isoform 1. This sequence region is 100% conserved among all known isoforms of human YAP protein.

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