Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein, PeproTech®

应用及建议稀释比

免疫印迹 (WB)：Assay-dependent

酶联免疫吸附实验 (ELISA)：Assay-dependent

免疫沉淀 (IP)：-

功能检测 (Functional)：Assay-dependent

放射免疫测定 (RIA)

对照试剂 (Ctrl)

体外检测 (IV)

其他PubMed (Misc)

免疫染色 (IS)

产品规格

种属：Human

已发表种属：Amphibian, Avian, Bacteria, Bovine, Camelid, Cat, Chicken, Cynomolgus monkey, Dog, Fish, Guinea pig, Hamster, Horse, Human, Mammal, Mouse, Non-human primate, Panda, Pig, Rabbit, Rat, Sheep, Virus

Expression System：E. coli

氨基酸序列：AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

分子量：17.2 kDa

分类：Recombinant

类型：Protein

纯度：≥ 95% by SDS-PAGE gel and HPLC analyses.

内毒素浓度：<1 EU/µg

活性：Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

偶联物：Unconjugated

形式：Lyophilized

纯化类型：purified

内含物：no preservative

保存条件：-20°C

运输条件：Ambient

产品详细信息

Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues. Qualified for stem cell use.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

靶标信息

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。