Anti-BIP1/2 /Heat shock 70 kDa protein rabbit antibody
Antibody Type
Primary Antibodies
Immunogen
Recombinant protein of Arabidopsis thaliana At5g42020
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:2500-1:5000
Species Reactivity
ArabidopsisRice
Purification
Affinity purified
Target Information
Gene Symbol
BIP
Gene Synonyms
At5g42020, At5g28540, LOC_Os02g02410
Gene Full Name
heat shock protein 70 (Hsp 70) family protein
Gene Summary
Encodes the luminal binding protein BiP, an ER-localized member of the HSP70 family. BiP is composed of an N-terminal ATP binding domain and a C-terminal domain that binds to hydrophobic patches on improperly/incompletely folded proteins in an ATP-dependent manner. Involved in polar nuclei fusion during proliferation of endosperm nuclei.
Molecular Weight(MW)
74 kDa for Arabidopsis thaliana
Application
WB
Sample loading: Approximately 2 μg of total proteins from Arabidopsis thaliana and rice leaves. Electrophoresis: 12.5% SDS - PAGE. Membrane transfer: Nitrocellulose (NC) membrane, transfer at 10 V overnight. Blocking: Block with 5% non - fat dry milk at room temperature for 1 hour. Primary antibody: Dilute the primary antibody at a ratio of 1:5000 and incubate at room temperature for 1 hour.
