Lysine malonylation is a recently discovered reversible modification that can regulate protein activity. Sirt5 is a lysine malonylation modifying enzyme that catalyzes the deprotonation of lysine both in vivo and in vitro. Integrating proteomics and biochemical analysis techniques, it has been found that lysine malonylation can occur in both prokaryotic and eukaryotic organisms, regardless of whether they are histones or non histones. Lysine malonylation may provide a new paradigm for studying intracellular regulation such as energy metabolism in various organisms.
Application
WB
Primary antibody dilution: 1:1000, primary antibody incubation conditions: room temperature for 2 hours, secondary antibody: HRP labeled Goat Anti Mouse IgG (H+L) at 1:1000 dilution. Lysate: JK, 293T protein loading volume: 20 μ g Exposure time: 60 seconds Theoretical molecular weight: Multiple kDa, Actual molecular weight: Multiple kDa.
Application Notes
WB:1:500-1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at -20 º C and avoid repeated freezing and thawing.
