| 存储条件 |
|---|
| Product Name | Cleaved PARP1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:5000 IHC:1:200-1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PARP1 |
|---|---|
| Gene Synonyms | PARP PARS PPOL ADPRT ARTD1 ADPRT1 PARP-1 ADPRT 1 pADPRT-1 Poly-PARP |
| Gene Full Name | poly(ADP-ribose) polymerase 1 |
| Gene Summary | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008] |
| Alternative Names | PARP1 ADPRT PPOL Poly [ADP-ribose] polymerase 1 PARP-1 ADP-ribosyltransferase diphtheria toxin-like 1 ARTD1 NAD + ADP-ribosyltransferase 1 ADPRT 1 Poly[ADP-ribose] synthase 1 |
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Cytoplasmic, Nuclear. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved PARP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 Lane 2: Hela Predicted band size: 25kDa.
IHC
Rat colon tissue was stained with Anti-Cleaved PARP1 rabbit Antibody.
IHC
Human tonsil tissue was stained with Anti-Cleaved PARP1 rabbit Antibody.
WB
a Western blot analysis of Cleaved-Caspase3 and Cleaved-PARP protein expression, and both of them were enhanced following TNF-α treatment. GAPDH was used as a loading control.
WB
a Western blot analysis of Cleaved-Caspase3 and Cleaved-PARP protein expression indicated that apoptosis was significantly inhibited by knocking down TIPE2. GAPDH was used as a loading control.| Application Notes | WB:1:500-1:5000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
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