| 存储条件 |
|---|
| Product Name | CLPP Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CLPP aa 228-277 / 277. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:1000 ICC/IF:1:100 FC:1:500-1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CLPP |
|---|---|
| Gene Synonyms | DFNB81 PRLTS3 |
| Gene Full Name | caseinolytic mitochondrial matrix peptidase proteolytic subunit |
| Gene Summary | The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATP dependent protease ClpAP (E coli) proteolytic subunit antibody ATP dependent protease ClpAP proteolytic subunit antibody ATP dependent protease ClpAP proteolytic subunit human antibody ATP dependent proteolytic subunit homolog (E coli) antibody ATP dependent proteolytic subunit homolog antibody Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody Caseinolytic protease ATP dependent proteolytic subunit E coli antibody clpP antibody ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody ATP dependent protease ClpAP (E coli) proteolytic subunit antibody ATP dependent protease ClpAP proteolytic subunit antibody ATP dependent protease ClpAP proteolytic subunit human antibody ATP dependent proteolytic subunit homolog (E coli) antibody ATP dependent proteolytic subunit homolog antibody Caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody Caseinolytic protease ATP dependent proteolytic subunit E coli antibody clpP antibody ClpP caseinolytic peptidase ATP dependent proteolytic subunit antibody ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog antibody CLPP_HUMAN antibody Endopeptidase Clp antibody mitochondrial antibody Putative ATP dependent Clp protease proteolytic subunit mitochondrial antibody Putative ATP dependent Clp protease proteolytic subunit antibody Putative ATP-dependent Clp protease proteolytic subunit antibody |
| Molecular Weight(MW) | 30kDa(Observed band size:25kDa) |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: K-562 cell lysate (20 µg/Lane), Lane 3: SW620 cell lysate (20 µg/Lane), Lane 4: Jurkat cell lysate (20 µg/Lane), Lane 5: HepG2 cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Lane 8: Mouse heart tissue lysate (40 µg/Lane), Lane 9: Rat heart tissue lysate (39 µg/Lane), Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CLPP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:2000 IHC:1:1000 ICC/IF:1:100 FC:1:500-1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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