| 存储条件 |
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| Product Name | Wilms Tumor Protein Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human WT1 aa 59-269 / 449. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:2000-1:5000 IHC:1:500-1:1000 ICC:1:100-1:200 IF-P:1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WT1 |
|---|---|
| Gene Synonyms | GUD AWT1 WAGR WT-1 WT33 NPHS4 WIT-2 |
| Gene Full Name | WT1 transcription factor |
| Gene Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015] |
| Alternative Names | WIT 2 antibody WT 1 antibody AWT1 antibody FWT1 antibody GUD antibody NPHS4 antibody WAGR antibody Wilms tumor 1 antibody Wilms Tumor antibody Wilms tumor protein antibody WIT 2 antibody WT 1 antibody AWT1 antibody FWT1 antibody GUD antibody NPHS4 antibody WAGR antibody Wilms tumor 1 antibody Wilms Tumor antibody Wilms tumor protein antibody Wilms' tumor gene antibody Wilms' tumor protein antibody WIT2 antibody WT antibody WT1 antibody WT1_HUMAN antibody WT33 antibody |
| Molecular Weight(MW) | 49kDa |
| Cellular Localization | Nucleus, Cytoplasm, Nucleus speckle. |
WB
Western blot analysis of Wilms Tumor Protein on different lysates with Rabbit anti-Wilms Tumor Protein antibody at 1/2,000 dilution. Lane 1: K-562 cell lysate, Lane 2: Mouse testis tissue lysate, Lane 2: Rat testis tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-Wilms Tumor Protein antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of K-562 cells labeling Wilms Tumor Protein with Rabbit anti-Wilms Tumor Protein antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Wilms Tumor Protein antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling Wilms Tumor Protein with Rabbit anti-Wilms Tumor Protein antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:2000-1:5000 IHC:1:500-1:1000 ICC:1:100-1:200 IF-P:1:200 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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