| 存储条件 |
|---|
| Product Name | Ctip1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Ctip1 aa 381-609 / 835. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:800 ICC:1:100 FC:1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL11A |
|---|---|
| Gene Synonyms | EVI9 CTIP1 DILOS ZNF856 HBFQTL5 SMARCM1 |
| Gene Full Name | BCL11 transcription factor A |
| Gene Summary | This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 2810047E18Rik antibody B cell CLL/lymphoma 11A (zinc finger protein) antibody B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody B-cell CLL/lymphoma 11A antibody B-cell lymphoma/leukemia 11A antibody BC11A_HUMAN antibody BCL-11A antibody BCL11A antibody BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody BCL11A L antibody 2810047E18Rik antibody B cell CLL/lymphoma 11A (zinc finger protein) antibody B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody B-cell CLL/lymphoma 11A antibody B-cell lymphoma/leukemia 11A antibody BC11A_HUMAN antibody BCL-11A antibody BCL11A antibody BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody BCL11A L antibody BCL11A S antibody BCL11A XL antibody BCL11a-M antibody BCL11AL antibody BCL11AS antibody BCL11AXL antibody C2H2 type zinc finger protein antibody COUP TF interacting protein 1 antibody COUP-TF-interacting protein 1 antibody CTIP1 antibody CTIP1 mouse homolog of antibody D930021L15Rik antibody Ecotropic viral integration site 9 antibody Ecotropic viral integration site 9 homolog antibody Ecotropic viral integration site 9 protein antibody Ecotropic viral integration site 9 protein homolog antibody EVI-9 antibody Evi9 antibody Evi9 mouse homolog of antibody FLJ10173 antibody FLJ34997 antibody HBFQTL5 antibody KIAA1809 antibody mKIAA1809 antibody OTTHUMP00000159788 antibody OTTHUMP00000159789 antibody OTTHUMP00000201250 antibody OTTHUMP00000202084 antibody Zinc finger protein 856 antibody ZNF856 antibody |
| Molecular Weight(MW) | 91kDa(Observed band size: 120kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of Ctip1 on different lysates with Rabbit anti-Ctip1 antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate, Lane 2: Raji cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Ctip1 antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling Ctip1 with Rabbit anti-Ctip1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ctip1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of Jurkat cells labeling Ctip1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:5000 IHC:1:800 ICC:1:100 FC:1:1000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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