| 存储条件 |
|---|
| Product Name | IRG1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse IRG1 aa 1-488. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC:1:100 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Acod1 |
|---|---|
| Gene Synonyms | CAD IRG1 |
| Gene Full Name | aconitate decarboxylase 1 |
| Gene Summary | Enables aconitate decarboxylase activity and protein homodimerization activity. Involved in defense response; positive regulation of antimicrobial humoral response; and tolerance induction to lipopolysaccharide. Predicted to be active in mitochondrion. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | ACOD1 antibody Aconitate decarboxylase 1 antibody Aconitate decarboxylase antibody CAD antibody Cis aconitate decarboxylase antibody Immune responsive gene 1 protein homolog antibody Immune-responsive gene 1 protein homolog antibody Immunoresponsive 1 homolog (mouse) antibody Irg1 antibody IRG1_HUMAN antibody ACOD1 antibody Aconitate decarboxylase 1 antibody Aconitate decarboxylase antibody CAD antibody Cis aconitate decarboxylase antibody Immune responsive gene 1 protein homolog antibody Immune-responsive gene 1 protein homolog antibody Immunoresponsive 1 homolog (mouse) antibody Irg1 antibody IRG1_HUMAN antibody OC116 antibody OPTB1 antibody TCTIRC7 antibody |
| Molecular Weight(MW) | 54kDa |
| Cellular Localization | Mitochondrion. |
WB
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate, Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate, Lane 3: J774A.1 cell lysate, Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate, Lane 5: mBMMC cell lysate, Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate, Lane 7: mBMSC cell lysate, Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours labeling IRG1 with Rabbit anti-IRG1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRG1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
