| 存储条件 |
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| Product Name | CD34 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CD34 aa 336-385 / 385. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:2000 IHC:1:400-1:10000 ICC:1:100 FC:1:1000 IP:1-2μg/sample | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD34 |
|---|---|
| Gene Full Name | CD34 molecule |
| Gene Summary | The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Alternative Names | CD34 antibody CD34 antigen antibody CD34 molecule antibody CD34_HUMAN antibody Cluster designation 34 antibody Hematopoietic progenitor cell antigen CD34 antibody HPCA1 antibody Mucosialin antibody OTTHUMP00000034733 antibody OTTHUMP00000034734 antibody |
| Molecular Weight(MW) | 41kDa(Observed band size:100 kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of CD34 on TF-1 cell lysates with Rabbit anti-CD34 antibody at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of TF-1 cells labeling CD34 with Rabbit anti-CD34 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD34 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of TF-1 cells labeling CD34. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
CD34 was immunoprecipitated from 0.2 mg TF-1 cell lysate with Rabbit anti-CD34 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate usingRabbit anti-CD34 antibody at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: TF-1 cell lysate (input), Lane 2: Rabbit anti-CD34 antibody IP in TF-1 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CD34 antibody in TF-1 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 6 seconds.| Application Notes | WB:1:2000 IHC:1:400-1:10000 ICC:1:100 FC:1:1000 IP:1-2μg/sample |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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