| 存储条件 |
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| Product Name | Phospho-MEK1/2 (S218 + S222) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser218 and 222 of Human MEK1 aa 200-249 / 393. |
| Modification | p-S218 + S222 |
| Modification Notes | Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP2K1 |
|---|---|
| Gene Synonyms | MEL CFC3 MEK1 MKK1 MAPKK1 PRKMK1 |
| Gene Full Name | mitogen-activated protein kinase kinase 1 |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
| Alternative Names | Dual specificity mitogen activated protein kinase kinase 1 antibody Dual specificity mitogen-activated protein kinase kinase 1 antibody ERK activator kinase 1 antibody MAP kinase kinase 1 antibody MAP kinase/Erk kinase 1 antibody MAP2K1 antibody MAPK/ERK kinase 1 antibody MAPKK 1 antibody MAPKK1 antibody MEK 1 antibody Dual specificity mitogen activated protein kinase kinase 1 antibody Dual specificity mitogen-activated protein kinase kinase 1 antibody ERK activator kinase 1 antibody MAP kinase kinase 1 antibody MAP kinase/Erk kinase 1 antibody MAP2K1 antibody MAPK/ERK kinase 1 antibody MAPKK 1 antibody MAPKK1 antibody MEK 1 antibody Mek1 antibody MEKK1 antibody Mitogen activated protein kinase kinase 1 antibody MKK 1 antibody MKK1 antibody MP2K1_HUMAN antibody PRKMK1 antibody Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody Protein kinase mitogen activated kinase 1 antibody hide |
| Molecular Weight(MW) | 43kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Membrane, Nucleus. |
WB
Western blot analysis of Phospho-MEK1/2 (S218 + S222) on different lysates with Rabbit anti-Phospho-MEK1/2 (S218 + S222) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, Lane 3: C6 cell lysate, Lane 4: C6 treated with 200nM PMA for 30 minutes cell lysate, Lane 5: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lane 6: C6 treated with 200nM PMA for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Phospho-MEK1/2 (S218 + S222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells treated with 200nM PMA for 30 minutes labeling Phospho-MEK1/2 (S218 + S222) with Rabbit anti-Phospho-MEK1/2 (S218 + S222) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-MEK1/2 (S218 + S222) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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