| 存储条件 |
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| Product Name | LEF1 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human LEF1 aa 121-170 / 399. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000 IHC:1:500-1:2000 ICC:1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LEF1 |
|---|---|
| Gene Synonyms | LEF-1 TCF10 TCF7L3 TCF1ALPHA |
| Gene Full Name | lymphoid enhancer binding factor 1 |
| Gene Summary | This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| Alternative Names | DKFZp586H0919 antibody FLJ46390 antibody LEF 1 antibody LEF-1 antibody Lef1 antibody LEF1_HUMAN antibody Lymphoid enhancer binding factor 1 antibody Lymphoid enhancer-binding factor 1 antibody T cell specific transcription factor 1 alpha antibody T cell-specific transcription factor 1-alpha antibody DKFZp586H0919 antibody FLJ46390 antibody LEF 1 antibody LEF-1 antibody Lef1 antibody LEF1_HUMAN antibody Lymphoid enhancer binding factor 1 antibody Lymphoid enhancer-binding factor 1 antibody T cell specific transcription factor 1 alpha antibody T cell-specific transcription factor 1-alpha antibody TCF 1 alpha antibody TCF1 alpha antibody TCF1-alpha antibody TCF10 antibody TCF1alpha antibody TCF7L3 antibody Transcription factor T cell specific 1 alpha antibody |
| Molecular Weight(MW) | 44kDa(Observed band size: 35-55kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Lane 3: Mouse thymus tissue lysate (40 µg/Lane) Lane 4: Rat thymus tissue lysate (40 µg/Lane) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 IHC:1:500-1:2000 ICC:1:500 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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