| 存储条件 |
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| Product Name | Cdk9 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Cdk9 aa 323-372 / 372. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:50-1:1000 ICC:1:50-1:200 FC:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDK9 |
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| Gene Synonyms | TAK C-2k CTK1 CDC2L4 PITALRE |
| Gene Full Name | cyclin dependent kinase 9 |
| Gene Summary | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008] |
| Alternative Names | C-2K antibody CDC2 related kinase antibody CDC2L4 antibody Cdk 9 antibody Cdk9 antibody CDK9_HUMAN antibody Cell division cycle 2-like protein kinase 4 antibody Cell division protein kinase 9 antibody CTK1 antibody Cyclin dependent kinase 9 antibody C-2K antibody CDC2 related kinase antibody CDC2L4 antibody Cdk 9 antibody Cdk9 antibody CDK9_HUMAN antibody Cell division cycle 2-like protein kinase 4 antibody Cell division protein kinase 9 antibody CTK1 antibody Cyclin dependent kinase 9 antibody Cyclin-dependent kinase 9 antibody PITALRE antibody Serine/threonine-protein kinase PITALRE antibody TAK antibody Tat associated kinase complex catalytic subunit antibody |
| Molecular Weight(MW) | 43kDa(Observed band size: 38/50kDa) |
| Cellular Localization | Nucleus, PML body, Cytoplasm. |
WB
Western blot analysis of Cdk9 on different lysates with Rabbit anti-Cdk9 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: F9 cell lysate Lane 5: PC-12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Cdk9 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of Cdk9 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
FC
Flow cytometric analysis of C6 cells labeling Cdk9. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:50-1:1000 ICC:1:50-1:200 FC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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