| 存储条件 |
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| Product Name | BRG1 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human BRG1 aa 240-280. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-FIF-PIHCWB |
| WB:1:20000-1:50000 IHC:1:1000-1:5000 ICC:1:200-1:1000 IF-F:1:1000 IF-P:1:50 FC:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMARCA4 |
|---|---|
| Gene Synonyms | BRG1 CSS4 SNF2 SWI2 MRD16 RTPS2 BAF190 OTSC12 SNF2L4 SNF2LB hSNF2b BAF190A SNF2-beta |
| Gene Full Name | SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4 |
| Gene Summary | The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| Alternative Names | ATP dependent helicase SMARCA4 antibody ATP-dependent helicase SMARCA4 antibody BAF 190 antibody BAF190 antibody BAF190A antibody Brahma protein like 1 antibody BRG1 antibody BRG1 associated factor 190A antibody BRG1 protein antibody BRG1-associated factor 190A antibody ATP dependent helicase SMARCA4 antibody ATP-dependent helicase SMARCA4 antibody BAF 190 antibody BAF190 antibody BAF190A antibody Brahma protein like 1 antibody BRG1 antibody BRG1 associated factor 190A antibody BRG1 protein antibody BRG1-associated factor 190A antibody BRM/SWI2 related gene 1 antibody Global transcription activator homologous sequence antibody global transcription activator snf2l4 antibody Homeotic gene regulator antibody hSNF2b antibody Mitotic growth and transcription activator antibody MRD16 antibody Nuclear protein GRB1 antibody Protein brahma homolog 1 antibody Protein BRG-1 antibody Protein BRG1 antibody RTPS2 antibody SMARC A4 antibody SMARCA4 antibody SMCA4_HUMAN antibody SNF2 antibody SNF2 beta antibody SNF2 like 4 antibody SNF2-beta antibody SNF2B antibody SNF2L4 antibody SNF2LB antibody Sucrose nonfermenting like 4 antibody SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 antibody SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody SWI2 antibody Transcription activator BRG1 antibody |
| Molecular Weight(MW) | 185kDa(Observed band size: 200kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of BRG1 on different lysates with Rabbit anti-BRG1 antibody at 1/50,000 dilution . Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A549 cell lysate (negative) Lane 4: NIH/3T3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-BRG1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling BRG1 with Rabbit anti-BRG1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRG1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Immunofluorescence analysis of frozen mouse testis tissue with Rabbit anti-BRG1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling BRG1 with Rabbit anti-BRG1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
FC
Flow cytometric analysis of HeLa cells labeling BRG1. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-BRG1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BRG1 antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:20000-1:50000 IHC:1:1000-1:5000 ICC:1:200-1:1000 IF-F:1:1000 IF-P:1:50 FC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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