| 存储条件 |
|---|
| Product Name | S100A8 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human S100A8 aa 1-93/93 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:2000-1:5000 IHC:1:10000 ICC:1:50000 FC:1:1000 IP:1-2μg/sample | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | S100A8 |
|---|---|
| Gene Synonyms | P8 MIF NIF CAGA CFAG CGLA L1Ag MRP8 CP-10 MA387 60B8AG S100-A8 |
| Gene Full Name | S100 calcium binding protein A8 |
| Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| Alternative Names | Calgranulin-A antibody Calprotectin L1L subunit antibody CFAG antibody Cystic fibrosis antigen antibody Leukocyte L1 complex light chain antibody Migration inhibitory factor-related protein 8 antibody MRP-8 antibody N-terminally processed antibody p8 antibody Protein S100-A8 antibody Calgranulin-A antibody Calprotectin L1L subunit antibody CFAG antibody Cystic fibrosis antigen antibody Leukocyte L1 complex light chain antibody Migration inhibitory factor-related protein 8 antibody MRP-8 antibody N-terminally processed antibody p8 antibody Protein S100-A8 antibody S100 calcium-binding protein A8 antibody S100A8 antibody S10A8_HUMAN antibody Urinary stone protein band A antibody |
| Molecular Weight(MW) | 11kDa(Observed band size:14kDa) |
| Cellular Localization | Secreted; Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane. |
WB
Western blot analysis of S100A8 on different lysates with Rabbit anti-S100A8 antibody at 1/5,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (negative) (20 µg/Lane) Lane 3: HeLa cell lysate (negative) (20 µg/Lane) Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-S100A8 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling S100A8 with Rabbit anti-S100A8 antibody at 1/50,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100A8 antibody at 1/50,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of 293T (left, negative) and SK-Br-3 (right, positive) cells labeling S100A8. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
S100A8 was immunoprecipitated from 0.2 mg SK-Br-3 cell lysate with Rabbit anti-S100A8 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-S100A8 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-Br-3 cell lysate (input) Lane 2: Rabbit anti-S100A8 antibody IP in SK-Br-3 cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-S100A8 antibody in SK-Br-3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes.| Application Notes | WB:1:2000-1:5000 IHC:1:10000 ICC:1:50000 FC:1:1000 IP:1-2μg/sample |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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