| 存储条件 |
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| Product Name | AMPK beta 1 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human AMPK beta 1 aa 90-130. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKAB1 |
|---|---|
| Gene Synonyms | AMPK HAMPKb |
| Gene Full Name | protein kinase AMP-activated non-catalytic subunit beta 1 |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1300015D22Rik antibody 5'-AMP-activated protein kinase subunit beta-1 antibody 5'-AMP-activated protein kinase beta-1 subunit antibody AAKB1_HUMAN antibody AMP-activated protein kinase beta subunit antibody AMP-ACTIVATED PROTEIN KINASE NONCATALYTIC BETA-1 antibody AMP-activated noncatalytic beta-1 antibody AMPK antibody AMPK beta 1 chain antibody AMPK subunit beta-1 antibody 1300015D22Rik antibody 5'-AMP-activated protein kinase subunit beta-1 antibody 5'-AMP-activated protein kinase beta-1 subunit antibody AAKB1_HUMAN antibody AMP-activated protein kinase beta subunit antibody AMP-ACTIVATED PROTEIN KINASE NONCATALYTIC BETA-1 antibody AMP-activated noncatalytic beta-1 antibody AMPK antibody AMPK beta 1 chain antibody AMPK subunit beta-1 antibody AMPK-BETA-1 antibody AMPKb antibody AU021155 antibody E430008F22 antibody HAMPKb antibody MGC17785 antibody PRKAB1 antibody Protein kinase AMP activated non catalytic subunit beta 1 antibody protein kinase AMP-activated beta 1 non-catalytic subunit antibody protein kinase AMP-activated noncatalytic beta-1 antibody |
| Molecular Weight(MW) | 30kDa(Observed band size: 36kDa) |
| Cellular Localization | Cytosol, nucleoplasm, nucleus, cytoplasm, nucleotide-activated protein kinase complex. |
WB
Western blot analysis of AMPK beta 1 on different lysates with Rabbit anti-AMPK beta 1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using anti-AMPK beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of C2C12 cells labeling AMPK beta 1 with Rabbit anti-AMPK beta 1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMPK beta 1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of AMPK beta 1 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100 FC:1:50-1:100 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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