| 存储条件 |
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| Product Name | PSD95 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human PSD95 aa 1-40. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:2000 IHC:1:2000 IF-F:1:50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DLG4 |
|---|---|
| Gene Synonyms | MRD62 PSD95 SAP90 SAP-90 |
| Gene Full Name | discs large MAGUK scaffold protein 4 |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Discs large homolog 4 antibody Disks large homolog 4 antibody DLG 4 antibody Dlg4 antibody DLG4_HUMAN antibody FLJ97752 antibody FLJ98574 antibody Human post synaptic density protein 95 antibody Post synaptic density protein 95 antibody Postsynaptic density protein 95 antibody Discs large homolog 4 antibody Disks large homolog 4 antibody DLG 4 antibody Dlg4 antibody DLG4_HUMAN antibody FLJ97752 antibody FLJ98574 antibody Human post synaptic density protein 95 antibody Post synaptic density protein 95 antibody Postsynaptic density protein 95 antibody PSD 95 antibody PSD-95 antibody PSD95 antibody SAP 90 antibody SAP-90 antibody SAP90 antibody Synapse associated protein 90 antibody Synapse-associated protein 90 antibody Tax interaction protein 15 antibody |
| Molecular Weight(MW) | 80kDa(Observed band size: 75-100kDa) |
| Cellular Localization | Cell membrane, Postsynaptic density, Synapse, Cytoplasm, Cell projection, axon, dendritic spine, dendrite, Presynapse. |
WB
Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 80 kDa Observed band size: 75-100 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-PSD95 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-PSD95 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:2000 IHC:1:2000 IF-F:1:50 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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