| 存储条件 |
|---|
| Product Name | Beclin 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Beclin 1 aa 351-450 / 450. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:200 ICC:1:250 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BECN1 |
|---|---|
| Gene Synonyms | ATG6 VPS30 beclin1 |
| Gene Full Name | beclin 1 |
| Gene Summary | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Alternative Names | APG6 antibody ATG 6 antibody ATG6 antibody ATG6 autophagy related 6 homolog antibody Bcl-2-interacting protein beclin antibody Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody Beclin 1 autophagy related antibody Beclin-1 antibody Beclin1 antibody BECN 1 antibody APG6 antibody ATG 6 antibody ATG6 antibody ATG6 autophagy related 6 homolog antibody Bcl-2-interacting protein beclin antibody Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody Beclin 1 autophagy related antibody Beclin-1 antibody Beclin1 antibody BECN 1 antibody Becn1 antibody BECN1_HUMAN antibody Coiled coil myosin like BCL2 interacting protein antibody Coiled-coil myosin-like BCL2-interacting protein antibody GT197 antibody Protein GT197 antibody VPS 30 antibody VPS30 antibody |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Cytoplasm, Golgi apparatus, trans-Golgi network membrane, Endosome membrane, Endoplasmic reticulum membrane, Mitochondrion membrane, Endosome, Cytoplasmic vesicle, autophagosome. |
WB
Western blot analysis of Beclin 1 on different lysates with Rabbit anti-Beclin 1 antibody at 1/1,000 dilution. Lane 1: MG-63 cell lysate (20 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 3: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: Lane 1: 3 minutes; Lane 2-3: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Beclin 1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Raji cells labeling Beclin 1 with Rabbit anti-Beclin 1 antibody at 1/250 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beclin 1 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:500-1:1000 IHC:1:200 ICC:1:250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
抱歉，暂无浏览记录
