| 存储条件 |
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| Product Name | Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human SMAD1 and SMAD5 protein. |
| Modification | p-S463/S465/S467 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:1000 ICC:1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Smad1/5/9 |
|---|---|
| Gene Synonyms | BSP1 JV41 BSP-1 JV4-1 MADH1 MADR1 |
| Gene Full Name | SMAD family member 1/5/9 |
| Gene Summary | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Alternative Names | BSP-1 antibody BSP1 antibody HsMAD1 antibody JV4-1 antibody JV41 antibody MAD homolog 1 antibody MAD mothers against decapentaplegic homolog 1 antibody Mad related protein 1 antibody Mad-related protein 1 antibody MADH1 antibody BSP-1 antibody BSP1 antibody HsMAD1 antibody JV4-1 antibody JV41 antibody MAD homolog 1 antibody MAD mothers against decapentaplegic homolog 1 antibody Mad related protein 1 antibody Mad-related protein 1 antibody MADH1 antibody MADR1 antibody Mothers against decapentaplegic homolog 1 antibody Mothers against DPP homolog 1 antibody SMA- AND MAD-RELATED PROTEIN 1 antibody SMAD 1 antibody SMAD family member 1 antibody SMAD mothers against DPP homolog 1 antibody Smad1 antibody SMAD1_HUMAN antibody TGF beta signaling protein 1 antibody Transforming growth factor-beta-signaling protein 1 antibody DKFZp781C1895 antibody DKFZp781O1323 antibody Dwfc antibody hSmad5 antibody JV5 1 antibody JV5-1 antibody MAD homolog 5 antibody MAD mothers against decapentaplegic homolog 5 antibody MADH 5 antibody MADH5 antibody Mothers against decapentaplegic homolog 5 antibody mothers against decapentaplegic drosophila homolog of 5 antibody Mothers against DPP homolog 5 antibody MusMLP antibody SMA and MAD related protein 5 antibody SMAD 5 antibody SMAD family member 5 antibody SMAD mothers against DPP homolog 5 antibody Smad5 antibody SMAD5_HUMAN antibody MAD homolog 9 antibody Madh6 antibody Mothers against decapentaplegic antibody Mothers against decapentaplegic homolog 9 antibody Mothers against DPP homolog 9 antibody SMAD 9 antibody SMAD family member 9 antibody SMAD mothers against DPP homolog 9 (Drosophila) antibody SMAD8A antibody SMAD8B antibody Smad9 antibody SMAD9_HUMAN antibody |
| Molecular Weight(MW) | Predicted band size: 52 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Mitochondrion. |
WB
Western blot analysis of Phospho-Smad1/5/9 (S463/S465/S467) on different lysates with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 10ng/mL BMP4 for 1 hour cell lysate Lane 3: C6 treated with 10ng/mL BMP4 for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells treated with 10ng/mL BMP4 for 1 hour labeling Phospho-Smad1/5/9 (S463/S465/S467) with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Smad1/5/9 (S463/S465/S467) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:1000 ICC:1:100 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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