| 存储条件 |
|---|
| Product Name | GFAP Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C-terminal human GFAP. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:5000 IHC:1:500-1:1000 ICC:1:50-1:500 FC:1:50-1:100|IF-P | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GFAP |
|---|---|
| Gene Synonyms | ALXDRD |
| Gene Full Name | glial fibrillary acidic protein |
| Gene Summary | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Alternative Names | wu:fb34h11 antibody ALXDRD antibody cb345 antibody etID36982.3 antibody FLJ42474 antibody FLJ45472 antibody GFAP antibody GFAP_HUMAN antibody gfapl antibody Glial fibrillary acidic protein antibody wu:fb34h11 antibody ALXDRD antibody cb345 antibody etID36982.3 antibody FLJ42474 antibody FLJ45472 antibody GFAP antibody GFAP_HUMAN antibody gfapl antibody Glial fibrillary acidic protein antibody Intermediate filament protein antibody wu:fk42c12 antibody xx:af506734 antibody zgc:110485 antibody |
| Molecular Weight(MW) | Predicted band size: 50 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of GFAP on different lysates with Mouse anti-GFAP antibody at 1/5,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human cerebellum tissue lysate Lane 4: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GFAP antibody at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of GFAP was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
IF-P
Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling GFAP (EM140707) and NeuN (ET1602-12). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies GFAP (EM140707, Red) at 1/500 dilution and NeuN (ET1602-12, Green) at 1/50 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Rabbit IgG (HA1121) and iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) were used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.| Application Notes | WB:1:5000 IHC:1:500-1:1000 ICC:1:50-1:500 FC:1:50-1:100|IF-P |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
抱歉，暂无浏览记录
