| 存储条件 |
|---|
| Product Name | FKBP38 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human FKBP38 (N terminal) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 IHC:1:1000 ICC:1:100 FC:1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FKBP8 |
|---|---|
| Gene Synonyms | FKBP38 FKBPr38 |
| Gene Full Name | FKBP prolyl isomerase 8 |
| Gene Summary | The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Unlike the other members of the family, this encoded protein does not seem to have PPIase/rotamase activity. It may have a role in neurons associated with memory function. [provided by RefSeq, Jul 2008] |
| Alternative Names | 38 kDa FK506-binding protein antibody 38 kDa FKBP antibody 38kDa antibody FK506 binding protein 8 antibody FK506 binding protein 8 38kDa antibody FK506 binding protein 38kDa antibody FK506-binding protein 8 antibody FKBP 38 antibody FKBP-38 antibody FKBP-8 antibody 38 kDa FK506-binding protein antibody 38 kDa FKBP antibody 38kDa antibody FK506 binding protein 8 antibody FK506 binding protein 8 38kDa antibody FK506 binding protein 38kDa antibody FK506-binding protein 8 antibody FKBP 38 antibody FKBP-38 antibody FKBP-8 antibody Fkbp8 antibody FKBP8_HUMAN antibody FKBPR38 antibody hFKBP38 antibody Peptidyl-prolyl cis-trans isomerase FKBP8 antibody PPIase FKBP8 antibody Rotamase antibody Sam11 antibody |
| Molecular Weight(MW) | Predicted band size: 45 kDa(Observed band size: 55 kDa) |
| Cellular Localization | Mitochondrion, Mitochondrion membrane. |
| Entrez Gene | 23770 |
|---|
WB
Western blot analysis of FKBP38 on different lysates with Rabbit anti-FKBP38 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 55 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-FKBP38 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyat 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling FKBP38 with Rabbit anti-FKBP38 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FKBP38 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa cells labeling FKBP38. Cells were fixed and permeabilized. Then stained with the primary antibody ( 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 ICC:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
